rabbit anti timp3 polyclonal antibody  (Bioss)

Journal: World Journal of Hepatology

Article Title: Tissue inhibitor of metalloproteinase-3 expression affects clinicopathological features and prognosis of aflatoxin B1-related hepatocellular carcinoma

doi: 10.4254/wjh.v16.i8.1131

Figure Lengend Snippet: Effects of aflatoxin B1 exposure and tissue inhibitor of metalloproteinase-3 expression on survival of patients with aflatoxin B1-related hepatocellular carcinoma. A and B: Effects of aflatoxin B1 (AFB1) exposure on overall survival (OS) and tumor recurrence-free survival (RFS) of patients with AFB1-related hepatocellular carcinoma (AHCC). The level of AFB1 exposure was assessed using AFB1-DNA adducts in HCC cancerous tissues; C and D: Effects of TIMP3 expression on OS and RFS of patients with AHCC. The level of TIMP3 protein expression in AHCC tissues was tested by immunohistochemistry combined with the immunoreactive scoring system; E and F: Effects of AFB1 exposure combined with TIMP3 expression on OS and RFS of AHCC. Cumulative hazard function was plotted by the Kaplan-Meier method, and P value was calculated with two-sided log-rank tests. LAE: Low AFB1-exposure group; HAE: High AFB1-exposure group; LTE: Low TIMP3 expression; HTE: High TIMP3 expression; AETE1: Low aflatoxin B1 exposure plus high TIMP3 expression; AETE2: Low aflatoxin B1 exposure plus low TIMP3 expression; AETE3: High aflatoxin B1 exposure plus high TIMP3 expression; AETE4: High aflatoxin B1 exposure plus low TIMP3 expression.

Article Snippet: After that, TIMP3 protein was tested using an anti-TIMP3 polyclonal antibody (1:250 dilution; catalog bs-0417R, Beijing Bioss Biology, Inc., Beijing, China) and UltraSensitive TM SP IHC Kit (catalog KIT-9730, Maixin Biotechnology, Inc., Xiamen, China).

Techniques: Expressing, Immunohistochemistry

Journal: World Journal of Hepatology

Article Title: Tissue inhibitor of metalloproteinase-3 expression affects clinicopathological features and prognosis of aflatoxin B1-related hepatocellular carcinoma

doi: 10.4254/wjh.v16.i8.1131

Figure Lengend Snippet: Univariable Kaplan-Meier survival analysis

Article Snippet: After that, TIMP3 protein was tested using an anti-TIMP3 polyclonal antibody (1:250 dilution; catalog bs-0417R, Beijing Bioss Biology, Inc., Beijing, China) and UltraSensitive TM SP IHC Kit (catalog KIT-9730, Maixin Biotechnology, Inc., Xiamen, China).

Techniques:

Journal: World Journal of Hepatology

Article Title: Tissue inhibitor of metalloproteinase-3 expression affects clinicopathological features and prognosis of aflatoxin B1-related hepatocellular carcinoma

doi: 10.4254/wjh.v16.i8.1131

Figure Lengend Snippet: Univariable Cox proportional hazard regression survival analysis

Article Snippet: After that, TIMP3 protein was tested using an anti-TIMP3 polyclonal antibody (1:250 dilution; catalog bs-0417R, Beijing Bioss Biology, Inc., Beijing, China) and UltraSensitive TM SP IHC Kit (catalog KIT-9730, Maixin Biotechnology, Inc., Xiamen, China).

Techniques:

Journal: World Journal of Hepatology

Article Title: Tissue inhibitor of metalloproteinase-3 expression affects clinicopathological features and prognosis of aflatoxin B1-related hepatocellular carcinoma

doi: 10.4254/wjh.v16.i8.1131

Figure Lengend Snippet: Mulvariable Cox proportional hazard regression analysis for survival

Article Snippet: After that, TIMP3 protein was tested using an anti-TIMP3 polyclonal antibody (1:250 dilution; catalog bs-0417R, Beijing Bioss Biology, Inc., Beijing, China) and UltraSensitive TM SP IHC Kit (catalog KIT-9730, Maixin Biotechnology, Inc., Xiamen, China).

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Marek's Disease Virus and Reticuloendotheliosis Virus Coinfection Enhances Viral Replication and Alters Cellular Protein Profiles

doi: 10.3389/fvets.2022.854007

Figure Lengend Snippet: Confirmation of four differentially expressed proteins (IRF7, MX1, AKT1, and TIMP3) in MDV and REV coinfected and single infected, and mock-infected CEF cells by Western blotting. β-Actin was used as an internal control to normalize the quantitative data. MDV, Marek's disease virus; REV, reticuloendotheliosis virus; CEF, chicken embryo fibroblast.

Article Snippet: The membranes were incubated at 37°C for 60 min with rabbit polyclonal antibody to TIMP3 (Abcam, Cambridge, UK), rabbit polyclonal antibody to AKT1 (BIOSS, Beijing, China), rabbit polyclonal antibody to MX1 (ProteinTech Group, Chicago, IL, USA), rabbit polyclonal antibody to IRF7 (BIOSS, Beijing, China), mouse monoclonal antibody anti-REV gp90, and rabbit polyclonal anti-MDV pp38.

Techniques: Infection, Western Blot, Control, Virus

Journal: Cancer cell

Article Title: CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes

doi: 10.1016/j.ccell.2017.04.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-TIMP3 , Thermo Fisher , Cat#PA1-21146; RRID: AB_561715.

Techniques: Recombinant, Transfection, Protease Inhibitor, SYBR Green Assay, Reverse Transcription, Isolation, DNA Methylation Assay, DNA Purification, Chromatin Immunoprecipitation, CRISPR, Methylation, Software

Journal: Oncotarget

Article Title: Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition

doi: 10.18632/oncotarget.16657

Figure Lengend Snippet: ( A ) KG1a and NB4 cells were treated with DMSO (Ctrl) or DAC (0.25, 0.5 and 1 μM) for 48 hours, and TIMP3 expression was analyzed by qRT-PCR. Each bar represents the relative expression of TIMP3 normalized with respect to the reference gene (GAPDH), using the 2 −ΔCt method. MICA transcription levels in the KG1a cell line untreated (DMSO, Ctrl) or treated with DAC at different concentrations were used as a positive control. Results are summarized as the mean ± SEM of five independent experiments. * p < 0.05 and * p < 0.01. ( B ) TIMP3 protein levels were evaluated by western blot in KG1a and NB4 cells after treatment with DMSO (Ctrl) or DAC (1 μM or 5 μM) for 48 hours. * p < 0.05. ( C ) The TIMP3 methylation pattern was quantified by pyrosequencing in AML cell lines (KG1a and NB4 cells) before and after treatment with 1 μM or 5 μM DAC. Pie charts show the average percentage of methylation for the CpGs analyzed in the TIMP3 gene. ( D ) TIMP3 expression was inhibited by transfection of KG1a cells previously treated with DAC (1 μM) with a TIMP3-specific siRNA or nonspecific scramble siRNA (200 nM). * p < 0.05 ( E ) Soluble NKG2DL were quantified by sandwich ELISA after TIMP3 inhibition. Values shown are the mean ± SEM of three independent experiments. * versus control and p < 0.05; * versus control and p < 0.01 # versus nonspecific scramble siRNA and p < 0.05.

Article Snippet: After blocking, primary polyclonal goat anti-human TIMP3 and ß-actin (Santa Cruz Biotechnology, Dallas, TX) were incubated overnight at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Positive Control, Western Blot, Methylation, Transfection, Sandwich ELISA, Inhibition, Control

Journal: Oncotarget

Article Title: Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition

doi: 10.18632/oncotarget.16657

Figure Lengend Snippet: ( A ) The TIMP3 DNA methylation levels were quantified by pyrosequencing in bone marrow cells obtained at diagnosis from 90 AML patients and from 25 healthy donors. Each sample is represented by a black dot. ( B ) Pie charts indicating the frequency of AML patients with aberrant DNA methylation levels (> 20%) for TIMP3 by cytogenetic prognosis group (favorable, intermediate and adverse) of AML patients.

Article Snippet: After blocking, primary polyclonal goat anti-human TIMP3 and ß-actin (Santa Cruz Biotechnology, Dallas, TX) were incubated overnight at 4°C.

Techniques: DNA Methylation Assay, Biomarker Discovery

Journal: Oncotarget

Article Title: Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition

doi: 10.18632/oncotarget.16657

Figure Lengend Snippet: Genetic alterations of AML patients in association with TIMP3 methylation

Article Snippet: After blocking, primary polyclonal goat anti-human TIMP3 and ß-actin (Santa Cruz Biotechnology, Dallas, TX) were incubated overnight at 4°C.

Techniques: Methylation